Anti-OTUB1 Antibody Picoband®

SKU: A04361-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: OTUB1

Specificity:

Background: Ubiquitin thioesterase OTUB1 also known as otubain-1 is an enzyme that in humans is encoded by the OTUB1 gene. The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human OTUB1 recombinant protein (Position: K10-K271). Human OTUB1 shares 99.6% amino acid (aa) sequence identity with both mouse and rat OTUB1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Balakirev, M. Y., Tcherniuk, S. O., Jaquinod, M., Chroboczek, J. Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep. 4: 517-522, 2003. 2. Lin, J. T., Lineberry, N. B., Kattah, M. G., Su, L. L., Utz, P. J., Fathman, C. G., Wu, L. Naive CD4 T cell proliferation is controlled by mammalian target of rapamycin regulation of GRAIL expression. J. Immun. 182: 5919-5928, 2009. 3. Nakada, S., Tai, I., Panier, S., Al-Hakim, A., Iemura, S., Juang, Y.-C., O'Donnell, L., Kumakubo, A., Munro, M., Sicheri, F., Gingras, A.-C., Natsume, T., Suda, T., Durocher, D. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. Nature 466: 941-946, 2010

Uniprot ID: Q96FW1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55611

Protein Name: Ubiquitin thioesterase OTUB1

Gene Full Name: OTU deubiquitinase, ubiquitin aldehyde binding 1

Synonyms: OTUB1; OTB1; OTU1; HSPC263; Ubiquitin thioesterase OTUB1; Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1