Anti-NUDT15 Antibody Picoband®

SKU: A05498-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUDT15

Specificity:

Background: Nudix hydrolase 15 is a protein that in humans is encoded by the NUDT15 gene. This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.

Immunogen: E.coli-derived human NUDT15 recombinant protein (Position: C23-L164). Human NUDT15 shares 90.8% amino acid (aa) sequence identity with mouse NUDT15.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cai, J.-P., Ishibashi, T., Takagi, Y., Hayakawa, H., Sekiguchi, M. Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine nucleotides. Biochem. Biophys. Res. Commun. 305: 1073-1077, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/13/2014. 3. Moriyama, T., Nishii, R., Perez-Andreu, V., Yang, W., Klussmann, F. A., Zhao, X., Lin, T.-N., Hoshitsuki, K., Nersting, J., Kihira, K., Hofmann, U., Komada, Y., and 22 others. NUDT15 polymorphisms alter thiopurine metabolism and hematopoietic toxicity. Nature Genet. 48: 367-373, 2016

Uniprot ID: Q9NV35

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 19 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55270

Protein Name: Nucleotide triphosphate diphosphatase NUDT15

Gene Full Name: nudix hydrolase 15

Synonyms: 8 oxo dGTPase NUDT15; MTH2; MutT homolog 2; Nudix motif 15; NUDT15