Anti-NSMCE4A Antibody Picoband®

SKU: A14289-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSMCE4A

Specificity:

Background: Non-SMC element 4 homolog A is a protein that in humans is encoded by the NSMCE4A gene. NSMCE4A is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). It is involved in positive regulation of response to DNA damage stimulus.

Immunogen: E.coli-derived human NSMCE4A recombinant protein (Position: D69-Q380).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hu, B., Liao, C., Millson, S. H., Mollapour, M., Prodromou, C., Pearl, L. H., Piper, P. W., Panaretou, B. Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex. Molec. Microbiol. 55: 1735-1750, 2005. 2. Taylor, E. M., Copsey, A. C., Hudson, J. J. R., Vidot, S., Lehmann, A. R. Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. Molec. Cell. Biol. 28: 1197-1206, 2008.

Uniprot ID: Q9NXX6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 54780

Protein Name: Non-structural maintenance of chromosomes element 4 homolog A

Gene Full Name: NSE4A component of SMC5/6 complex

Synonyms: NSMCE4A; C10orf86; PP4762; Non-structural maintenance of chromosomes element 4 homolog A; NS4EA; Non-SMC element 4 homolog A