Anti-NIT2 Antibody Picoband®

SKU: A05891-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NIT2

Specificity:

Background: NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

Immunogen: E.coli-derived human NIT2 recombinant protein (Position: M1-K249).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Asakura, M., Nakano, M., Hayashida, K., Fujii, H., Nakajima, M., Atsuda, K., Itoh, T., Fujiwara, R. Human nitrilase-like protein does not catalyze the hydrolysis of vildagliptin. Drug Metab. Pharmacokinet. 29: 463-469, 2014. 2. Chien, C.-H., Gao, Q.-Z., Cooper, A. J. L., Lyu, J.-H., Sheu, S.-Y. Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. J. Biol. Chem. 287: 25715-25726, 2012. 3. Jaisson, S., Veiga-da-Cunha, M., Van Schaftingen, E. Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2. Biochimie 91: 1066-1071, 2009.

Uniprot ID: Q9NQR4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight:

Gene ID: 56954

Protein Name: Omega-amidase NIT2

Gene Full Name: nitrilase family member 2

Synonyms: NIT2; CUA002; Omega-amidase NIT2; Nitrilase homolog 2