Anti-NDUFAF4 Antibody Picoband®

SKU: A11650-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NDUFAF4

Specificity:

Background: NADH:ubiquinone oxidoreductase complex assembly factor 4, (NDUFAF4) also known as Hormone-regulated proliferation-associated protein of 20 kDa, (HRPAP20) or C6orf66 is a protein that in humans is encoded by the NDUFAF4 gene. NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.

Immunogen: E.coli-derived human NDUFAF4 recombinant protein (Position: K24-K175).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baertling, F., Sanchez-Caballero, L., van den Brand, M. A. M., Wintjes, L. T., Brink, M., van den Brandt, F. A., Wilson, C., Rodenburg, R. J. T., Nijtmans, L. G. J. NDUFAF4 variants are associated with Leigh syndrome and cause a specific mitochondrial complex I assembly defect. Europ. J. Hum. Genet. 25: 1273-1277, 2017. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/8/2008. 3. Karp, C. M., Pan, H., Zhang, M., Buckley, D. J., Schuler, L. A., Buckley, A. R. Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells. Cancer Res. 64: 1016-1025, 2004.

Uniprot ID: Q9P032

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 21 kDa

Calculated Molecular Weight:

Gene ID: 29078

Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4

Gene Full Name: NADH:ubiquinone oxidoreductase complex assembly factor 4

Synonyms: C6orf66; HRPAP20; HSPC125; My013; NDUFAF4