Anti-MSH2 Antibody Picoband®

SKU: A00140-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MSH2

Specificity:

Background: DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms aheterodimer with MSH6 to make the human MutSα mismatch repair complex. It also dimerizes with MSH3 to form the MutSβ DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.

Immunogen: E.coli-derived human MSH2 recombinant protein (Position: Q337-T934).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. de Wind N, Dekker M, Berns A, Radman M, te Riele H (July 1995). Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer. Cell 82 (2): 321–30. 2. Mellon I, Rajpal DK, Koi M, Boland CR, Champe GN (April 1996). Transcription-coupled repair deficiency and mutations in human mismatch repair genes. Science 272 (5261): 557–60. 3. Wada-Hiraike, O., Yano, T., Nei, T., Matsumoto, Y., Nagasaka, K., Takizawa, S., Oishi, H., Arimoto, T., Nakagawa, S., Yasugi, T., Kato, S., Taketani, Y. The DNA mismatch repair gene hMSH2 is a potent coactivator of oestrogen receptor-alpha. Brit. J. Cancer 92: 2286-2291, 2005.

Uniprot ID: P43246

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 105 kDa

Calculated Molecular Weight: 89838 MW

Gene ID: 4436

Protein Name: DNA mismatch repair protein Msh2

Gene Full Name: mutS homolog 2

Synonyms: COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MSH2; MutS protein homolog 2