Anti-MMS19 Antibody Picoband®

SKU: A06218-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: ELISA,IF,IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MMS19

Specificity:

Background: MMS19 nucleotide excision repair protein homolog is a protein that in humans is encoded by the MMS19 gene. MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Immunogen: E.coli-derived human MMS19 recombinant protein (Position: E239-R998).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gari, K., Leon Ortiz, A. M., Borel, V., Flynn, H., Skehel, J. M., Boulton, S. J. MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism. Science 337: 243-245, 2012. 2. Hatfield, M. D., Reis, A. M. C., Obeso, D., Cook, J. R., Thompson, D. M., Rao, M., Friedberg, E. C., Queimado, L. Identification of MMS19 domains with distinct functions in NER and transcription. DNA Repair 5: 914-924, 2006. 3. Ito, S., Tan, L. J., Andoh, D., Narita, T., Seki, M., Hirano, Y., Narita, K., Kuraoka, I., Hiraoka, Y., Tanaka, K. MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation. Molec. Cell 39: 632-640, 2010.

Uniprot ID: Q96T76

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 113 kDa

Calculated Molecular Weight: 49655 MW

Gene ID: 64210

Protein Name: MMS19 nucleotide excision repair protein homolog

Gene Full Name: MMS19 cytosolic iron-sulfur assembly component

Synonyms: hMMS19; MET18; MET18 homolog; MMS19; MMS19 like protein; MMS19L