Anti-LRRN1 Antibody Picoband®

SKU: A12074-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LRRN1

Specificity:

Background: LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.

Immunogen: E.coli-derived human LRRN1 recombinant protein (Position: T74-R655).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Liu, B., Zhang, Y., Fan, Y., Wang, S., Li, Z., Deng, M., ... & Qu, X. (2019). Leucine‐rich repeat neuronal protein‐1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL. Cancer Science, 110(7), 2145-2155. 2. Liao, C. H., Wang, Y. H., Chang, W. W., Yang, B. C., Wu, T. J., Liu, W. L., ... & Yu, J. (2018). Leucine-rich repeat neuronal protein 1 regulates differentiation of embryonic stem cells by post-translational modifications of pluripotency factors. Stem Cells, 36(10), 1514-1524. 3. Hossain, M. S., Ozaki, T., Wang, H., Nakagawa, A., Takenobu, H., Ohira, M., ... & Nakagawara, A. (2008). N-MYC promotes cell proliferation through a transactivation of neuronal leucine-rich repeat protein-1 (NLRR1) gene in neuroblastoma. Oncogene, 27(46), 6075-6082.

Uniprot ID: Q6UXK5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 57633

Protein Name: Leucine-rich repeat neuronal protein 1

Gene Full Name: leucine rich repeat neuronal 1

Synonyms: LRRN1; KIAA1497; Nbla10449; UNQ693/PRO1338; Leucine-rich repeat neuronal protein 1; Neuronal leucine-rich repeat protein 1; NLRR-1