Anti-Lipocalin 2/LCN2 Antibody

SKU: A00452

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse

Applications: ELISA

Application Details: ELISA Capture (Matched Antibody Pair), 2-8μg/ml

Gene Name: Lcn2

Specificity: No cross reactivity with other proteins.

Background: Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Immunogen: E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cowland JB, Borregaard N (October 1997). Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans. Genomics 45 (1): 17–23. 2. Chan P, Simon-Chazottes D, Mattei MG, Guenet JL, Salier JP (September 1994). Comparative mapping of lipocalin genes in human and mouse: the four genes for complement C8 gamma chain, prostaglandin-D-synthase, oncogene-24p3, and progestagen-associated endometrial protein map to HSA9 and MMU2. Genomics 23 (1): 145–50. 3. Flo TH, Smith KD, Sato S, Rodriguez DJ, Holmes MA, Strong RK, Akira S, Aderem A (December 2004). Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron. Nature 432 (7019): 917–21. 4. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (May 1993). Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J. Biol. Chem. 268 (14): 10425–32. 5. Nelson AM, Zhao W, Gilliland KL, Zaenglein AL, Liu W, Thiboutot DM (April 2008). 6. Schmidt-Ott KM, Mori K, Li JY, Kalandadze A, Cohen DJ, Devarajan P, Barasch J (February 2007). Dual action of neutrophil gelatinase-associated lipocalin. J. Am. Soc. Nephrol. 18 (2): 407–13.

Uniprot ID: P11672

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 86 kDa

Calculated Molecular Weight: 22875 MW

Gene ID: 16819

Protein Name: Neutrophil gelatinase-associated lipocalin

Gene Full Name: lipocalin 2

Synonyms: lipocalin 2; 24p3; MSFI; NGAL; p25; Lcn2