Anti-Ifngr1 Antibody Picoband®

SKU: A01716-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Ifngr1

Specificity:

Background: Interferon gamma receptor 1 (IFNGR1) also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells. IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation. Due to differences in the degree of glycosylation, there are 3 forms of IFN-γ, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE. The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1. The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis.

Immunogen: E.coli-derived human PADI3 recombinant protein (Position: E24-R557). Human PADI3 shares 85.8% and 86.5% amino acid (aa) sequence identity with mouse and rat PADI3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aguet, M., Dembic, Z., Merlin, G. Molecular cloning and expression of the human interferon-gamma receptor. Cell 55: 273-280, 1988. 2. Alcaide-Loridan, C., Le Coniat, M., Bono, R., Benech, P., Couillin, P., Van Cong, N., Fisher, D. N., Berger, R., Fellous, M. Mapping of the human interferon gamma response. (Abstract) Cytogenet. Cell Genet. 51: 949 only, 1989. 3. Altare, F., Jouanguy, E., Lamhamedi-Cherradi, S., Fondaneche, M.-C., Fizame, C., Ribierre, F., Merlin, G., Dembic, Z., Schreiber, R., Lisowska-Grospierre, B., Fischer, A., Seboun, E., Casanova, J.-L. A causative relationship between mutant IFNgR1 alleles and impaired cellular response to IFN-gamma in a compound heterozygous child. (Letter) Am. J. Hum. Genet. 62: 723-726, 1998.

Uniprot ID: P15261

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 96 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 15979

Protein Name: Dedicator of cytokinesis protein 4 isoform X2

Gene Full Name: interferon gamma receptor 1

Synonyms: Ifngr1; Ifngr; Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; Interferon gamma receptor alpha-chain; IFN-gamma-R-alpha; CD antigen CD119