Anti-HSPA4L Antibody Picoband®

SKU: A10753-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,ICC,IHC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HSPA4L

Specificity:

Background: Heat shock 70 kDa protein 4L is a protein that in humans is encoded by the HSPA4L gene. The protein encoded by this gene is heat shock inducible and may act as a chaperone. The encoded protein can protect the heat-shocked cell against the harmful effects of aggregated proteins. This gene is highly expressed in leukemia cells and may be a good target for therapeutic intervention. Several transcripts encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human HSPA4L recombinant protein (Position: Q55-K788). Human HSPA4L shares 93.1% amino acid (aa) sequence identity with mouse HSPA4L.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Held, T., Paprotta, I., Khulan, J., Hemmerlein, B., Binder, L., Wolf, S., Schubert, S., Meinhardt, A., Engel, W., Adham, I. M. Hspa4l-deficient mice display increased incidence of male infertility and hydronephrosis development. Molec. Cell. Biol. 26: 8099-8108, 2006. 2. Liu, X., Wang, X., Liu, F. Decreased expression of heat shock protein A4L in spermatozoa is positively related to poor human sperm quality. Molec. Reprod. Dev. 86: 379-386, 2019. 3. Mohamed, B. A., Barakat, A. Z., Held, T., Elkenani, M., Muhlfeld, C., Manner, J., Adham, I. M. Respiratory distress and early neonatal lethality in Hspa4l/Hspa4 double-mutant mice. Am. J. Resp. Cell Molec. Biol. 50: 817-824, 2014.

Uniprot ID: O95757

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 22824

Protein Name: Heat shock 70 kDa protein 4L

Gene Full Name: heat shock protein family A (Hsp70) member 4 like

Synonyms: HSPA4L; APG1; OSP94; Heat shock 70 kDa protein 4L; Heat shock 70-related protein APG-1; Osmotic stress protein 94