Anti-HSPA4 Antibody Picoband®

SKU: A03618-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: HSPA4

Specificity:

Background: Heat shock 70 kDa protein 4 is a protein that in humans is encoded by the HSPA4 gene. The heat shock protein HSPA4 (Apg-2, HSP70RY) is a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins. Apg-2 has chaperone ability similiar to Hsp110, and it plays a role under non-stress conditions. Apg-2 interacts with TJP1/ZO-1 and functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA4, which shares 95.2% amino acid (aa) sequence identity with both mouse and rat HSPA4.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fathallah, D. M., Cherif, D., Dellagi, K., Arnaout, M. A. Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5. J. Immun. 151: 810-813, 1993. Note: Erratum: J. Immun. 151: 6616 only, 1993. 2. Gross, M. B. Personal Communication. Baltimore, Md. 10/22/2020. 3. Mohamed, B. A., Barakat, A. Z., Held, T., Elkenani, M., Muhlfeld, C., Manner, J., Adham, I. M. Respiratory distress and early neonatal lethality in Hspa4l/Hspa4 double-mutant mice. Am. J. Resp. Cell Molec. Biol. 50: 817-824, 2014.

Uniprot ID: P34932

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 110 kDa

Calculated Molecular Weight: 96,14 kDa

Gene ID: 3308

Protein Name: Heat shock 70 kDa protein 4

Gene Full Name: heat shock protein family A (Hsp70) member 4

Synonyms: APG 2; APG2; Heat shock 70 kDa protein 4; heat shock 70kDa protein 4; HS24/P52; HSP70.4; hsp70RY; HSPA4; RY