Anti-HEC1/HEC/NDC80 Antibody Picoband®

SKU: A01731-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: NDC80

Specificity: No cross reactivity with other proteins.

Background: Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. It is mapped to 18p11.32. This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Immunogen: E.coli-derived human HEC1/HEC/NDC80 recombinant protein (Position: N87-E602).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chen Y, Riley DJ, Chen PL, Lee WH (October 1997). HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Molecular and Cellular Biology. 17 (10): 6049–56. 2. Martin-Lluesma S, Stucke VM, Nigg EA (September 2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science. 297 (5590): 2267–70. 3. Chen Y, Sharp ZD, Lee WH (September 1997). HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins. The Journal of Biological Chemistry. 272 (38): 24081–7.

Uniprot ID: O14777

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 74 kDa

Calculated Molecular Weight: 57954 MW

Gene ID: 10403

Protein Name: Kinetochore protein NDC80 homolog

Gene Full Name: NDC80 kinetochore complex component

Synonyms: HEC; HEC1; HsHec1; hsNDC80; Kinetochore protein Hec1; KNTC2; NDC80; TID3