Anti-H-ERG/KCNH2 Antibody Picoband®

SKU: A00781-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: KCNH2

Specificity:

Background: KCNH2, also known as HERG or KV11.1, encodes the pore-forming subunit of a rapidly activating-delayed rectifier potassium channel. It is mapped to 7q36.1. KCNH2 forms the major portion of one of the ion channel proteins (the 'rapid' delayed rectifier current (IKr)) that conducts potassium (K+) ions out of the muscle cells of the heart (cardiac myocytes), and this current is critical in correctly timing the return to the resting state (repolarization) of the cell membrane during the cardiac action potential. What’s more, KCNH2 channels show gating properties consistent with many of the outwardly rectifying potassium channels, but they also have an inactivation mechanism that attenuates efflux during depolarization.

Immunogen: E.coli-derived human H-ERG/KCNH2 recombinant protein (Position: A121-V1074).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gianulis, E. C., Trudeau, M. C. Rescue of aberrant gating by a genetically encoded PAS (Per-Arnt-Sim) domain in several long QT syndrome mutant human ether-a-go-go-related gene potassium channels. J. Biol. Chem. 286: 22160-22169, 2011. 2. Hedley PL; Jorgensen P; Schlamowitz S; Wangari, Romilda et al. (2009). The genetic basis of long QT and short QT syndromes: a mutation update. Human Mutation 30 (11): 1486–511. 3. Thomas, P. J., Qu, B.-H., Pedersen, P. L. Defective protein folding as a basis of human disease. Trends Biochem. Sci. 20: 456-459, 1995.

Uniprot ID: Q12809

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 3757

Protein Name: Voltage-gated inwardly rectifying potassium channel KCNH2

Gene Full Name: potassium voltage-gated channel subfamily H member 2

Synonyms: KCNH2; ERG1; HERG; ERG-1; Eag-related protein 1; H-ERG; hERG-1