Anti-GTF2B Antibody Picoband®

SKU: A06893

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GTF2B

Specificity:

Background: This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Immunogen: E.coli-derived human GTF2B recombinant protein (Position: N17-D298). Human GTF2B shares 100% amino acid (aa) sequence identity with both mouse and rat GTF2B.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abendroth, F. D., Peterson, S. R., Galman, M., Suwa, A., Hardin, J. A., Dynan, W. S. Identification of human autoantibodies to transcription factor IIB. Nucleic Acids Res. 23: 2770-2774, 1995. 2. Bagby, S., Kim, S., Maldonado, E., Tong, K. I., Reinberg, D., Ikura, M. Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein. Cell 82: 857-867, 1995. 3. Bushnell, D. A., Westover, K. D., Davis, R. E., Kornberg, R. D. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 angstroms. Science 303: 983-988, 2004.

Uniprot ID: Q00403

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 35 kDa

Gene ID: 2959

Protein Name: Transcription initiation factor IIB

Gene Full Name: general transcription factor IIB

Synonyms: GTF2B; S300 II; TF2B; TFIIB