Anti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody

SKU: A01462-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA

Application Details: ELISA Capture (Matched Antibody Pair), 2-8μg/ml

Gene Name: GSTA1

Specificity: No cross reactivity with other proteins.

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules). In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity, thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Immunogen: E. coli-derived humn GSTA1/A2/A3/A4/A5 recombinant protein (Position: A2-F222).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Atkinson, HJ; Babbitt, PC (Nov 24, 2009). Glutathione transferases are structural and functional outliers in the thioredoxin fold.. Biochemistry 48 (46): 11108–16. 2. Boyer TD (March 1989). The glutathione S-transferases: an update. Hepatology 9(3): 486–96.

Uniprot ID: P08263

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 26 kDa

Calculated Molecular Weight: 25722 MW

Gene ID: 2938

Protein Name: Glutathione S-transferase A1

Gene Full Name: glutathione S-transferase alpha 1

Synonyms: Glutathione S transferase A1; GST class alpha member 1; GST epsilon; GST HA subunit 1; GST2; GSTA1; GSTA1 1; GTH1