Anti-Growth hormone receptor/GHR Antibody Picoband®

SKU: PA1726

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat

Gene Name: GHR

Specificity: No cross reactivity with other proteins.

Background: The GHR locus to human chromosome 5p13.1-p12 and to mouse chromosome 15. Additionally, its gene has 9 exons that encode the receptor and several additional exons in the 5-prime untranslated region. The coding exons span at least 87 kb. GHR consists of an extracellular domain of 246 amino acids, a single transmembrane domain, and a cytoplasmic domain. Exons 3 to 7 encode the extracellular domain. There are 2 isoforms of GHR in humans, generated by retention or exclusion of exon 3 during splicing: a full-length isoform and an isoform that lacks exon 3 (d3GHR). Furthermore, the two isoforms of GHR are expressed in the placenta and appeared to be due to alternative splicing. In cirrhosis, there is a state of acquired GH resistance, as defined by high circulating GH levels with low IGF1 levels. Moreover, Mutations in the GHR gene have been demonstrated as the cause of Laron syndrome , also known as the growth hormone insensitivity syndrome (GHIS).

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Growth hormone receptor, identical to the related rat sequence.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Barton, D. E.; Foellmer, B. E.; Wood, W. I.; Francke, U. : Chromosome mapping of the growth hormone receptor gene in man and mouse. Cytogenet. Cell Genet. 50: 137-141, 1989. 2. Godowski, P. J.; Leung, D. W.; Meacham, L. R.; Galgani, J. P.; Hellmiss, R.; Keret, R.; Rotwein, P. S.; Parks, J. S.; Laron, Z.; Wood, W. I. : Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism. Proc. Nat. Acad. Sci. 86: 8083-8087, 1989. 3. Amselem, S.; Sobrier, M.-L.; Duquesnoy, P.; Rappaport, R.; Postel-Vinay, M.-C.; Gourmelen, M.; Dallapiccola, B.; Goossens, M. : Recurrent nonsense mutations in the growth hormone receptor from patients with Laron dwarfism. J. Clin. Invest. 87: 1098-1102, 1991.

Uniprot ID: P16882

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight: 72783 MW

Gene ID: 14600

Protein Name: Growth hormone receptor

Gene Full Name: growth hormone receptor

Synonyms: GH binding protein; GH receptor; GHBP; GHR; Serum binding protein; Somatotropin receptor