Anti-GLIS3 Antibody Picoband®

SKU: A03710-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml/ml, Human

Gene Name: GLIS3

Specificity:

Background: Zinc finger protein GLI3 is a protein that in humans is encoded by the GLI3 gene. This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.

Immunogen: E.coli-derived human GLIS3 recombinant protein (Position: A184-P765).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Beak, J. Y., Kang, H. S., Kim, Y.-S., Jetten, A. M. Functional analysis of the zinc finger and activation domains of Glis3 and mutant Glis3(NDH1). Nucleic Acids Res. 36: 1690-1702, 2008. 2. Dimitri, P., Habeb, A. M., Gurbuz, F., Millward, A., Wallis, S., Moussa, K., Akcay, T., Taha, D., Hogue, J., Slavotinek, A., Wales, J. K. H., Shetty, A., Hawkes, D., Hattersley, A. T., Ellard, S., De Franco, E. Expanding the clinical spectrum associated with GLIS3 mutations. J. Clin. Endocr. Metab. 100: E1362-E1369, 2015. Note: Electronic Article. Erratum: J. Clin. Endocr. Metab. 100: 4685 only, 2015. 3. Dimitri, P., Warner, J. T., Minton, J. A. L., Patch, A. M., Ellard, S., Hattersley, A. T., Barr, S., Hawkes, D., Wales, J. K., Gregory, J. W. Novel GLIS3 mutations demonstrate an extended multisystem phenotype. Europ. J. Endocr. 164: 437-443, 2011.

Uniprot ID: Q8NEA6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 84 kDa

Calculated Molecular Weight: 26934 MW

Gene ID: 169792

Protein Name: Zinc finger protein GLIS3

Gene Full Name: GLIS family zinc finger 3

Synonyms: GLI similar 3; GLIS family zinc finger 3; GLIS3; Zinc finger protein 515; Zinc finger protein GLIS3; ZNF515