Anti-GANAB Antibody Picoband®

SKU: A09668-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GANAB

Specificity:

Background: Neutral alpha-glucosidase AB is an enzyme that in humans is encoded by the GANAB gene. This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease.

Immunogen: E.coli-derived human GANAB recombinant protein (Position: Q68-R944). Human GANAB shares 92% amino acid (aa) sequence identity with mouse GANAB.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Besse, W., Choi, J., Ahram, D., Mane, S., Sanna-Cherchi, S., Torres, V., Somlo, S. A noncoding variant in GANAB explains isolated polycystic liver disease (PCLD) in a large family. Hum. Mutat. 39: 378-382, 2018. 2. Martiniuk, F., Smith, M., Desnick, R., Astrin, K., Mitra, J., Hirschhorn, R. Assignment of the gene for neutral alpha-glucosidase AB to chromosome 11. (Abstract) Am. J. Hum. Genet. 34: 173A only, 1982. 3. Martiniuk, F., Smith, M., Ellenbogen, A., Desnick, R. J., Astrin, K., Mitra, J., Hirschhorn, R. Assignment of the gene for neutral alpha-glucosidase AB to chromosome 11. Cytogenet. Cell Genet. 35: 110-116, 1983.

Uniprot ID: Q14697

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight: 107 kDa

Gene ID: 23193

Protein Name: Neutral alpha-glucosidase AB

Gene Full Name: glucosidase II alpha subunit

Synonyms: GANAB; G2AN; KIAA0088; Neutral alpha-glucosidase AB; EC 3.2.1.207; Alpha-glucosidase 2; Glucosidase II subunit alpha