Anti-EVL Antibody Picoband®

SKU: A02568-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: EVL

Specificity:

Background: Ena/VASP-like protein is a member of the Ena/VASP family of proteins that in humans is encoded by the EVL gene. Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells. The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization. Research studies have shown that EVL also regulates DNA repair by interaction with RAD51. EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination. Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer.

Immunogen: E.coli-derived human EVL recombinant protein (Position: M268-E400).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 4/19/2016. 2. Janssens, K., De Kimpe, L., Balsamo, M., Vandoninck, S., Vandenheede, J. R., Gertler, F., Van Lint, J. Characterization of EVL-I as a protein kinase D substrate. Cell. Signal. 21: 282-292, 2009. 3. Klostermann, A., Lutz, B., Gertler, F., Behl, C. The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A-1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein-like protein (EVL) via a novel carboxyl-terminal zyxin-like domain. J. Biol. Chem. 275: 39647-39653, 2000.

Uniprot ID: Q9UI08

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight:

Gene ID: 51466

Protein Name: Ena/VASP-like protein

Gene Full Name: Enah/Vasp-like

Synonyms: Ena/VASP like protein; Enah/Vasp like; EVL; RNB6