Anti-ENPP1/PC1 Antibody Picoband®

SKU: A01342-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ENPP1

Specificity:

Background: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (PC-1, CD203a) is an enzyme that in humans is encoded by the ENPP1 gene. This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance.

Immunogen: E.coli-derived human ENPP1/PC1 recombinant protein (Position: E347-N789).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abate, N., Carulli, L., Cabo-Chan, Jr., A., Chandalia, M., Snell, P. G., Grundy, S. M. Genetic polymorphism PC-1 K121Q and ethnic susceptibility to insulin resistance. J. Clin. Endocr. Metab. 88: 5927-5934, 2003. 2. Bottcher, Y., Korner, A., Reinehr, T., Enigk, B., Kiess, W., Stumvoll, M., Kovacs, P. ENPP1 variants and haplotypes predispose to early onset obesity and impaired glucose and insulin metabolism in German obese children. J. Clin. Endocr. Metab. 91: 4948-4952, 2006. 3. Buckley, M. F., Goding, J. W. Plasma cell membrane glycoprotein gene Pca-1 (alkaline phosphodiesterase I) is linked to the proto-oncogene Myb on mouse chromosome 10. Immunogenetics 36: 199-201, 1992.

Uniprot ID: P22413

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130-150 kDa

Calculated Molecular Weight:

Gene ID: 5167

Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Gene Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 1

Synonyms: ENPP1; M6S1; NPPS; PC1; PDNP1; E-NPP1