Anti-EIF4A1 Antibody Picoband® (monoclonal, 11B8)

SKU: M03922-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat

Gene Name: EIF4A1

Specificity:

Background: Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EIF4A1, identical to the related mouse and rat sequences.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R. The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13. Genomics 53: 248-250, 1998. 3. Kim, N.-S., Kato, T., Abe, N., Kato, S. Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4A1. Nucleic Acids Res. 21: 2012 only, 1993.

Uniprot ID: P60842

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 11B8

Observed Molecular Weight: 46 kDa

Calculated Molecular Weight:

Gene ID: 1973

Protein Name: Eukaryotic initiation factor 4A-I

Gene Full Name: eukaryotic translation initiation factor 4A1

Synonyms: Eukaryotic initiation factor 4A-I; eIF-4A-I; EIF4A-I; ATP-dependent RNA helicase eIF4A-1; EIF4A1; DDX2A; EIF4A