Anti-DR3/TNFRSF25 Antibody Picoband®

SKU: PA2004

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: TNFRSF25

Specificity: No cross reactivity with other proteins.

Background: TNFRSF25 (Tumor Necrosis Factor Receptor Superfamily Member 25), also known as LARD, APO3, DR3 or TNFR25, is a protein that in humans is encoded by the TNFRSF25 gene. Members of the mammalian tumor necrosis factor receptor (TNFR) family are cell-surface proteins that interact with a corresponding TNF-related ligand family. By fluorescence in situ hybridization, Marsters et al. (1996) mapped the Apo3 gene to 1p36.3. Marsters et al. (1996) showed that ectopic expression of Apo3 in mammalian cells triggered apoptosis and activated the transcription factor NF-kappa-B. They suggested that, like TNFR1, Apo3 may regulate distinct signaling pathways in different cellular contexts.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DR3.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Borysenko, C. W., Furey, W. F., Blair, H. C. Comparative modeling of TNFRSF25 (DR3) predicts receptor destabilization by a mutation linked to rheumatoid arthritis. Biochem. Biophys. Res. Commun. 328: 794-799, 2005. 2. Fang, L., Adkins, B., Deyev, V., Podack, E. R. Essential role of TNF receptor superfamily 25 (TNFRSF25) in the development of allergic lung inflammation. J. Exp. Med. 205: 1037-1048, 2008. 3. Marsters, S. A., Sheridan, J. P., Donahue, C. J., Pitti, R. M., Gray, C. L., Goddard, A. D., Bauer, K. D., Ashkenazi, A. Apo-3, a new member of the tumor necrosis factor receptor family, contains a death domain and activates apoptosis and NF-kappa-B. Curr. Biol. 6: 1669-1676, 1996.

Uniprot ID: Q93038

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 59 kDa

Calculated Molecular Weight: 45385 MW

Gene ID: 8718

Protein Name: Tumor necrosis factor receptor superfamily member 25

Gene Full Name: TNF receptor superfamily member 25

Synonyms: APO 3; APO3; DDR3; Death receptor 3; DR3; LARD; Protein WSL; Protein WSL 1; TNFRSF12; TNFRSF25; TR3; TRAMP; WSL; WSL 1; WSL LR; WSL1