Anti-DGKZ/DGK-zeta Antibody Picoband®

SKU: A06678-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: DGKZ

Specificity:

Background: Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.

Immunogen: E.coli-derived human DGKZ/DGK-zeta recombinant protein (Position: E664-V928).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bunting, M., Tang, W., Zimmerman, G. A., McIntyre, T. M., Prescott, S. M. Molecular cloning and characterization of a novel human diacylglycerol kinase. J. Biol. Chem. 271: 10230-10236, 1996. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/11/2014. 3. Topham, M. K., Bunting, M., Zimmerman, G. A., McIntyre, T. M., Blackshear, P. J., Prescott, S. M. Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta. Nature 394: 697-700, 1998.

Uniprot ID: Q13574

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 124 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 8525

Protein Name: Diacylglycerol kinase zeta

Gene Full Name: diacylglycerol kinase zeta

Synonyms: diacylglycerol kinase zeta; DAGK5; DAGK6; DGK-ZETA; hDGKzeta; DGK-zeta; DGKZ