Anti-DFFA/ICAD Antibody Picoband®

SKU: A03671-4

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: DFFA

Specificity:

Background: DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen: E.coli-derived human DFFA/ICAD recombinant protein (Position: E14-S308).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Abel, F., Sjoberg, R.-M., Ejeskar, K., Krona, C., Martinsson, T. Analyses of apoptotic regulators CASP9 and DFFA at 1p36.2, reveal rare allele variants in human neuroblastoma tumours. Brit. J. Cancer 86: 596-604, 2002. 2. Inohara, N., Koseki, T., Chen, S., Wu, X., Nunez, G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 17: 2526-2533, 1998. 3. Leek, J. P., Carr, I. M., Bell, S. M., Markham, A. F., Lench, N. J. Assignment of the DNA fragmentation factor gene (DFFA) to human chromosome bands 1p36.3-p36.2 by in situ hybridization. Cytogenet. Cell Genet. 79: 212-213, 1997.

Uniprot ID: O00273

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 28461 MW

Gene ID: 1676

Protein Name: DNA fragmentation factor subunit alpha

Gene Full Name: DNA fragmentation factor subunit alpha

Synonyms: DFF 45; DFF1; DFF45; DFF45/DFFA; DFFA; ICAD; Inhibitor of CAD