Anti-DCC Antibody Picoband®

SKU: A00560

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IHC,WB

Application Details: Western blot, 0.1-0.25μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: DCC

Specificity: No cross reactivity with other proteins.

Background: Deleted in Colorectal Carcinoma, also known as DCC, is a protein which in humans is encoded by the DCC gene. It is mapped to 18q21.2. This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.

Immunogen: E.coli-derived human DCC recombinant protein (Position: Q851-F1447).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chan, S. S.-Y., Zheng, H., Su, M.-W., Wilk, R., Killeen, M. T., Hedgecock, E. M., Culotti, J. G. UNC-40, a C. elegans homolog of the DCC (deleted in colorectal cancer), is required in motile cells responding to UNC-6 netrin cues. Cell 87: 187-195, 1996. 2. Cho, K. R., Oliner, J. D., Simons, J. W., Hedrick, L., Fearon, E. R., Preisinger, A. C., Hedge, P., Silverman, G. A., Vogelstein, B. The DCC gene: structural analysis and mutations in colorectal carcinomas. Genomics 19: 525-531, 1994.

Uniprot ID: P43146

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 180 kDa

Calculated Molecular Weight: 9072 MW

Gene ID: 1630

Protein Name: Netrin receptor DCC

Gene Full Name: DCC netrin 1 receptor

Synonyms: Colorectal cancer suppressor; CRC18; CRCR1; DCC; IGDCC1; Netrin receptor DCC; Tumor suppressor protein DCC