Anti-DBF4 Antibody Picoband®

SKU: A01348-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry(Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: DBF4

Specificity:

Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

Immunogen: E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. 2. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. 3. Zegerman, P., Diffley, J. F. X. Checkpoint-dependent inhibition of DNA replication initiation by Sld3 and Dbf4 phosphorylation. Nature 467: 474-478, 2010.

Uniprot ID: Q9UBU7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight:

Gene ID: 10926

Protein Name: Protein DBF4 homolog A

Gene Full Name: DBF4-CDC7 kinase regulatory subunit

Synonyms: Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4A; Protein DBF4 homolog A; ZDBF1