Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

SKU: MA1026

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,IHC,WB

Application Details: Western blot, 0.5-1μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Immunofluorescence, 5 μg/ml, Human

Gene Name: KRT18

Specificity: No cross reactivity with other proteins.

Background: Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Immunogen: The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Ku, N.-O.; Wright, T. L.; Terrault, N. A.; Gish, R.; Omary, M. B. : Mutation of human keratin 18 in association with cryptogenic cirrhosis. J. Clin. Invest. 99: 19-23, 1997. 2. Waseem, A.; Gough, A. C.; Spurr, N. K.; Lane, E. B. : Localization of the gene for human simple epithelial keratin 18 to chromosome 12 using polymerase chain reaction. Genomics 7: 188-194, 1990.

Uniprot ID: Q5BJY9

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: CY-90

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight: 47761 MW

Gene ID: Human(3875), Mouse(16668), Rat(294853)

Protein Name: Keratin, type I cytoskeletal 18

Gene Full Name: keratin 18

Synonyms: K18; Keratin-18; CK-18; Cytokeratin-18; CYK18; Cell proliferation-inducing gene 46 protein