Anti-Cytokeratin 5/KRT5 Antibody Picoband®

SKU: A00398

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human

Gene Name: KRT5

Specificity: No cross reactivity with other proteins.

Background: Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: KRT5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types). 2. Eckert RL, Rorke EA (Jun 1988). The sequence of the human epidermal 58-kD (#5) type II keratin reveals an absence of 5' upstream sequence conservation between coexpressed epidermal keratins. Dna. 7 (5): 337–45. 3. Lersch R, Fuchs E (Jan 1988). Sequence and expression of a type II keratin, K5, in human epidermal cells. Molecular and Cellular Biology. 8 (1): 486–93.

Uniprot ID: P13647

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 62 kDa

Calculated Molecular Weight: 62378 MW

Gene ID: 3852

Protein Name: Keratin, type II cytoskeletal 5

Gene Full Name: keratin 5

Synonyms: 58 kDa cytokeratin; Cell and organelle markers; CK 5; CK5; Cytokeratin 5; Cytoskeleton Marker; K5; keratin 5; KRT5; KRT5A; Type II keratin Kb5