Anti-Cytokeratin 1/Krt1 Antibody Picoband®

SKU: A01639-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse
Immunofluorescence, 5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Krt1

Specificity:

Background: Keratin 1 (KRT1), also known as Cytokeratin 1, or Keratin, type II cytoskeletal 1, is a member of the keratin family. It is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants ofbullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. And this type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Immunogen: E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: KRT1 keratin 1 (epidermolytic hyperkeratosis). 2. Whittock NV, Ashton GH, Griffiths WA et al. (2001). New mutations in keratin 1 that cause bullous congenital ichthyosiform erythroderma and keratin 2e that cause ichthyosis bullosa of Siemens. Br. J. Dermatol. 145(2): 330–5.

Uniprot ID: P04104

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight: 141435 MW

Gene ID: 16678

Protein Name: Keratin, type II cytoskeletal 1

Gene Full Name: keratin 1

Synonyms: 67 kDa cytokeratin; CK 1; CK1; Cytokeratin 1; keratin 1; KRT1