Anti-CYC1 Antibody Picoband®

SKU: A02958-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CYC1

Specificity:

Background: Cytochrome c1, heme protein, mitochondrial (CYC1), also known as UQCR4, MC3DN6, Complex III subunit 4, Cytochrome b-c1 complex subunit 4, or Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit is a protein that in humans is encoded by the CYC1 gene. This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6.

Immunogen: E.coli-derived human CYC1 recombinant protein (Position: S85-K325).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Duncan, A. M. V., Ozawa, T., Suzuki, H., Rozen, R. Assignment of the gene for the cytochrome c1 subunit of the mitochondrial cytochrome bc-1 complex (CYC1) to human chromosome 8q24.3. Genomics 19: 400-401, 1994. 2. Fukuyama, R., Minoshima, S., Nishikimi, M., Ozawa, T., Suzuki, H., Shimizu, N. Assignment of cytochrome c1 gene to chromosome 8q24 distal to MYC. (Abstract) Cytogenet. Cell Genet. 58: 1929 only, 1991. 3. Gaignard, P., Menezes, M., Schiff, M., Bayot, A., Rak, M., Ogier de Baulny, H., Su, C.-H., Gilleron, M., Lombes, A., Abida, H., Tzagoloff, A., Riley, L., and 11 others. Mutations in CYC1, encoding cytochrome c1 subunit of respiratory chain complex III, cause insulin-responsive hyperglycemia. Am. J. Hum. Genet. 93: 384-389, 2013.

Uniprot ID: P08574

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight: 58026 MW

Gene ID: 1537

Protein Name: Cytochrome c1, heme protein, mitochondrial

Gene Full Name: cytochrome c1

Synonyms: Complex III subunit 4; Complex III subunit IV; CYC1; cytochrome c 1; UQCR4