Anti-Collagen VII/COL7A1 Antibody Picoband®

SKU: A01170-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: COL7A1

Specificity:

Background: Collagen alpha-1 (VII) chain is a protein that in humans is encoded by the COL7A1 gene. This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen.

Immunogen: E.coli-derived human Collagen VII/COL7A1 recombinant protein (Position: E2764-D2944).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Betts, C. M., Posteraro, P., Costa, A. M., Varotti, C., Schubert, M., Bruckner-Tuderman, L., Castiglia, D. Pretibial dystrophic epidermolysis bullosa: a recessively inherited COL7A1 splice site mutation affecting procollagen VII processing. Brit. J. Derm. 141: 833-839, 1999. 2. Christiano, A. M., Anhalt, G., Gibbons, S., Bauer, E. A., Uitto, J. Premature termination codons in the type VII collagen gene (COL7A1) underlie severe, mutilating recessive dystrophic epidermolysis bullosa. Genomics 21: 160-168, 1994. 3. Christiano, A. M., Hoffman, G. G., Chung-Honet, L. C., Lee, S., Cheng, W., Uitto, J., Greenspan, D. S. Structural organization of the human type VII collagen gene (COL7A1), composed of more exons than any previously characterized gene. Genomics 21: 169-179, 1994.

Uniprot ID: Q02388

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 300 kDa

Calculated Molecular Weight:

Gene ID: 1294

Protein Name: Collagen alpha-1

Gene Full Name: collagen type VII alpha 1 chain

Synonyms: COL7A1; Collagen alpha 1(VII) chain; Collagen Type VII; EBD1; EBDCT; EBR1; LC collagen; Long chain collagen