Anti-CDC45L Picoband® Antibody (monoclonal, 6H6)

SKU: M01367-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: CDC45

Specificity:

Background: CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Immunogen: E. coli-derived human CDC45L recombinant protein (Position: E166-A431).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: CDC45L CDC45 cell division cycle 45-like (S. cerevisiae). 2. McKie JM, Wadey RB, Sutherland HF, Taylor CL, Scambler PJ (Sep 1998). selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene. Genome Res. 8 (8): 834–41. 3. Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (Aug 1998). The human homolog of Saccharomyces cerevisiae CDC45. J. Biol. Chem. 273 (29): 18205–9.

Uniprot ID: O75419

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 6H6

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight:

Gene ID: 8318

Protein Name: Cell division control protein 45 homolog

Gene Full Name: cell division cycle 45

Synonyms: CDC45; CDC45L; CDC45L2; PORC PI 1