Anti-Cd63 Antibody Picoband®

SKU: A01080-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Cd63

Specificity:

Background: CD63 antigen is a protein that, in humans, is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

Immunogen: E.coli-derived mouse Cd63 recombinant protein (Position: A103-I203). Mouse Cd63 shares 67.3% and 93.1% amino acid (aa) sequence identity with human and rat Cd63, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gwynn, B., Eicher, E. M., Peters, L. L. Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals two distinct loci in the mouse genome. Genomics 35: 389-391, 1996. 2. Hotta, H., Ross, A. H., Huebner, K., Isobe, M., Wendeborn, S., Chao, M. V., Ricciardi, R. P., Tsujimoto, Y., Croce, C. M., Koprowski, H. Molecular cloning and characterization of an antigen associated with early stages of melanoma tumor progression. Cancer Res. 48: 2955-2962, 1988. 3. Metzelaar, M. J., Wijngaard, P. L. J., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., Clevers, H. C. CD63 antigen: a novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. J. Biol. Chem. 266: 3239-3245, 1991.

Uniprot ID: P41731

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 12512

Protein Name: CD63 antigen

Gene Full Name: CD63 antigen

Synonyms: CD63; CD63 antigen; CD63 molecule; Cell and organelle markers; Endosome Marker; Exosome marker; Granulophysin; LAMP 3; ME491; MLA1; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30