Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)

SKU: M01513-4

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,IHC-F

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human

Gene Name: PECAM1

Specificity: No cross reactivity with other proteins.

Background: CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Immunogen: E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Newman PJ, Berndt MC, Gorski J, White GC, Lyman S, Paddock C, Muller WA (March 1990). PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247 (4947): 1219–22. 2. Gumina RJ, Kirschbaum NE, Rao PN, vanTuinen P, Newman PJ (June 1996). The human PECAM1 gene maps to 17q23. Genomics 34 (2): 229–32. 3. Xie Y, Muller WA (October 1996). Fluorescence in situ hybridization mapping of the mouse platelet endothelial cell adhesion molecule-1 (PECAM1) to mouse chromosome 6, region F3-G1. Genomics 37 (2): 226–8. 4. Kellermair J, Redwan B, Alias S, Jabkowski J, Panzenboeck A, Kellermair L, Winter MP, Weltermann A, Lang IM.Platelet endothelial cell adhesion molecule 1 deficiency misguides venous thrombus resolution.2013 Nov 7; 122(19):3376-84. 5. Donmez A, Tombuloglu M, Gulbahar O, Arik B, Cagirgan S, Vural F, Gokmen N.CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments.2013 Oct;49(2):307-12.

Uniprot ID: P16284

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2D4

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 5175

Protein Name: Platelet endothelial cell adhesion molecule

Gene Full Name: platelet and endothelial cell adhesion molecule 1

Synonyms: CD31; EndoCAM; GPIIA; PECA1; PECAM 1; PECAM1; VEC marker