Anti-CD3 Gamma/CD3G Antibody Picoband®

SKU: A04853-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CD3G

Specificity:

Background: T-cell surface glycoprotein CD3 gamma chain is a protein that in humans is encoded by the CD3G gene. The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Immunogen: E.coli-derived human CD3 Gamma/CD3G recombinant protein (Position: Q23-D165).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Arnaiz-Villena, A., Perez-Aciego, P., Ballestin, C., Sotelo, T., Perez-Seoane, C., Martin-Villa, J. M., Regueiro, J. R. Biochemical basis of a novel T lymphocyte receptor immunodeficiency by immunohistochemistry: a possible CD3-gamma abnormality. Lab. Invest. 64: 675-681, 1991. 2. Arnaiz-Villena, A., Timon, M., Corell, A., Perez-Aciego, P., Martin-Villa, J. M., Regueiro, J. R. Primary immunodeficiency caused by mutations in the gene encoding the CD3-gamma subunit of the T-lymphocyte receptor. New Eng. J. Med. 327: 529-533, 1992. 3. Das, S., Cotter, F. E., Gibbons, B., Dhut, S., Young, B. D. CD3G is within 200 kb of the leukemic t(4;11) translocation breakpoint. Genes Chromosomes Cancer 3: 44-47, 1991.

Uniprot ID: P09693

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 20-25 kDa

Calculated Molecular Weight: 35883 MW

Gene ID: 917

Protein Name: T-cell surface glycoprotein CD3 gamma chain

Gene Full Name: CD3 gamma subunit of T-cell receptor complex

Synonyms: CD3 gamma; CD3G; CD3-gamma; T cell receptor T3 gamma chain; T3G