Anti-CD20 Antibody Picoband® (monoclonal, 4D11)

SKU: M03780-5

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,IF

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human

Gene Name: MS4A1

Specificity:

Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.

Immunogen: E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005). The biology of CD20 and its potential as a target for mAb therapy. Curr. Dir. Autoimmun. Current ions in Autoimmunity 8: 140–74. 2. Hardy, Richard (2008). Chapter 7: B Lymphocyte Development and Biology. In Paul, William.Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 237–269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010). CD20 deficiency in humans results in impaired T cell-independent antibody responses. J. Clin. Invest. 120 (1): 214–22.

Uniprot ID: P11836

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 4D11

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight:

Gene ID: 931

Protein Name: B-lymphocyte antigen CD20

Gene Full Name: membrane spanning 4-domains A1

Synonyms: B lymphocyte antigen CD20; B1; Bp35; CD20; LEU 16; MS4A1; MS4A2; S7