Anti-CD153/TNFSF8 Antibody

SKU: RP1103

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IHC

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x10⁶cells

Gene Name: TNFSF8

Specificity: No cross reactivity with other proteins.

Background: CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cerutti, A., Schaffer, A., Goodwin, R. G., Shah, S., Zan, H., Ely, S., Casali, P. Engagement of CD153 (CD30 ligand) by CD30-positive T cells inhibits class switch DNA recombination and antibody production in human IgD-positive IgM-positive B cells. J. Immun. 165: 786-794, 2000. 2. Croager, E. J., Abraham, L. J. Characterisation of the human CD30 ligand gene structure. Biochim. Biophys. Acta 1353: 231-235, 1997. 3. Fischer, M., Harvima, I. T., Carvalho, R. F. S., Moller, C., Naukkarinen, A., Enblad, G., Nilsson, G. Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion. J. Clin. Invest. 116: 2748-2756, 2006.

Uniprot ID: P32971

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 86 kDa

Calculated Molecular Weight: 26017 MW

Gene ID: 944

Protein Name: Tumor necrosis factor ligand superfamily member 8

Gene Full Name: TNF superfamily member 8

Synonyms: TNF superfamily member 8; CD153; CD30L; CD30LG; TNLG3A; TNFSF8