Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband®

SKU: A01083-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CA9

Specificity:

Background: Carbonic anhydrase IX (CA9/CA IX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Immunogen: E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hewett-Emmett, D., Tashian, R. E. Functional diversity, conservation, and convergence in the evolution of the alpha-, beta- and gamma- carbonic anhydrase gene families. Molec. Phylogenet. Evol. 5: 50-77, 1996. 2. Ivanov, S. V., Kuzmin, I., Wei, M.-H., Pack, S., Geil, L., Johnson, B. E., Stanbridge, E. J., Lerman, M. I. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes. Proc. Nat. Acad. Sci. 95: 12596-12601, 1998. 3. Nakagawa, Y., Uemura, H., Hirao, Y., Yoshida, K., Saga, S., Yoshikawa, K. Radiation hybrid mapping of the human MN/CA9 locus to chromosome band 9p12-p13. Genomics 53: 118-119, 1998.

Uniprot ID: Q16790

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight:

Gene ID: 768

Protein Name: Carbonic anhydrase 9

Gene Full Name: carbonic anhydrase 9

Synonyms: CA IX; CA9; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; carbonic anhydrase IX; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated antigen G250