Anti-CAMLG Antibody Picoband®

SKU: A08322-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: CAMLG

Specificity:

Background: The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CAMLG. Human CAMLG shares 91.7% and 95.7% amino acid (aa) sequence identity with mouse and rat CAMLG, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bram, R. J., Crabtree, G. R. Calcium signalling in T cells stimulated by a cyclophilin B-binding protein. Nature 371: 355-358, 1994. 2. Bram, R. J., Valentine, V., Shapiro, D. N., Jenkins, N. A., Gilbert, D. J., Copeland, N. G. The gene for calcium-modulating cyclophilin ligand (CAMLG) is located on human chromosome 5q23 and a syntenic region of mouse chromosome 13. Genomics 31: 257-260, 1996. 3. Guo, S., Lopez-Ilasaca, M., Dzau, V. J. Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation. J. Biol. Chem. 280: 12536-12541, 2005.

Uniprot ID: P49069

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 36 kDa

Gene ID: 819

Protein Name: Guided entry of tail-anchored proteins factor CAMLG

Gene Full Name: calcium modulating ligand

Synonyms: calcium modulating ligand; CAML; CAMLG