Anti-BCL3 Antibody Picoband®

SKU: A02773-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BCL3

Specificity:

Background: B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.

Immunogen: E.coli-derived human PELI2 recombinant protein (Position: D118-D420). Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bhatia, K., Huppi, K., McKeithan, T., Siwarski, D., Mushinski, J. F., Magrath, I. Mouse bcl-3: cDNA structure, mapping and stage-dependent expression in B lymphocytes. Oncogene 6: 1569-1573, 1991. 2. Carmody, R. J., Ruan, Q., Palmer, S., Hilliard, B., Chen, Y. H. Negative regulation of Toll-like receptor signaling by NF-kappa-B p50 ubiquitination blockade. Science 317: 675-678, 2007. 3. Franzoso, G., Bours, V., Park, S., Tomita-Yamaguchi, M., Kelly, K., Siebenlist, U. The candidate oncoprotein Bcl-3 is an antagonist of p50/NF-kappa-B-mediated inhibition. Nature 359: 339-342, 1992.

Uniprot ID: P20749

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 602

Protein Name: B-cell lymphoma 3 protein

Gene Full Name: BCL3 transcription coactivator

Synonyms: B cell CLL/lymphoma 3; B cell lymphoma 3 protein; BCL3; D19S37; Proto oncogene BCL3