Anti-Bag3 Antibody Picoband®

SKU: PB9754

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human

Gene Name: BAG3

Specificity: No cross reactivity with other proteins.

Background: BAG family molecular chaperone regulator 3 (BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men.

Immunogen: E.coli-derived human Bag3 recombinant protein (Position: H100-N561). Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Arndt V, Dick N, Tawo R, Dreiseidler M, Wenzel D, Hesse M, Fürst DO, Saftig P, Saint R, Fleischmann BK, Hoch M, Höhfeld J (January 2010). Chaperone-assisted selective autophagy is essential for muscle maintenance. Curr Biol 20 (2): 143–8. 2. Homma, S., Iwasaki, M., Shelton, G. D., Engvall, E., Reed, J. C., Takayama, S. BAG3 deficiency results in fulminant myopathy and early lethality. Am. J. Path. 169: 761-773, 2006. 3. Villard, E; Perret, C, Gary, F, Proust, C, Dilanian, G, Hengstenberg, C, Ruppert, V, Arbustini, E, Wichter, T, Germain, M, Dubourg, O, Tavazzi, L, Aumont, MC, DeGroote, P, Fauchier, L, Trochu, JN, Gibelin, P, Aupetit, JF, Stark, K, Erdmann, J, Hetzer, R, Roberts, AM, Barton, PJ, Regitz-Zagrosek, V; Cardiogenics Consortium, Aslam, U, Duboscq-Bidot, L, Meyborg, M, Maisch, B, Madeira, H, Waldenström, A, Galve, E, Cleland, JG, Dorent, R, Roizes, G, Zeller, T, Blankenberg, S, Goodall, AH, Cook, S, Tregouet, DA, Tiret, L, Isnard, R, Komajda, M, Charron, P, Cambien, F (April 1, 2011). A genome-wide association study identifies two loci associated with heart failure due to dilated cardiomyopathy 32 (9). pp. 1065–76.

Uniprot ID: O95817

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 61595 MW

Gene ID: 9531

Protein Name: BAG family molecular chaperone regulator 3

Gene Full Name: BAG cochaperone 3

Synonyms: BAG 3; BAG3; BIS; CAIR 1; Docking protein CAIR 1