Anti-ATP5F1,2,3/ATP5MC1,2,3 Picoband® Antibody (monoclonal, 12E9)

SKU: M09735

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat

Gene Name: ATP5MC1,2,3

Specificity:

Background: The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Immunogen: E.coli-derived human ATP5G1,2,3/ATP5MC1,2,3 recombinant protein (Position: D62-L113).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Dyer, M. R., Walker, J. E. Sequences of members of the human gene family for the c subunit of mitochondrial ATP synthase. Biochem. J. 293: 51-64, 1993. 2. Walker, J. E. Determination of the structures of respiratory enzyme complexes from mammalian mitochondria. Biochim. Biophys. Acta 1271: 221-227, 1995. 3. Yan, W. L., Lerner, T. J., Haines, J. L., Gusella, J. F. Sequence analysis and mapping of a novel human mitochondrial ATP synthase subunit 9 cDNA (ATP5G3). Genomics 24: 375-377, 1994.

Uniprot ID: P05496

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 12E9

Observed Molecular Weight: 10-14 kDa

Calculated Molecular Weight:

Gene ID: 516

Protein Name: ATP synthase F

Gene Full Name: ATP synthase membrane subunit c locus 1

Synonyms: ATP5MC1; ATP5G1; ATPase protein 9; ATPase subunit c; ATP5A; ATP5G