Anti-ATG12 Antibody Picoband®

SKU: A00820-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATG12

Specificity:

Background: Autophagy related 12 is a protein that in humans is encoded by the ATG12 gene. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Immunogen: E.coli-derived human ATG12 recombinant protein (Position: M68-Q99).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Mizushima, N., Sugita, H., Yoshimori, T., Ohsumi, Y. A new protein conjugation system in human: the counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273: 33889-33892, 1998. 2. Tanida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., Kominami, E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J. Biol. Chem. 277: 13739-13744, 2002. 3. Ueno, K., Kumagai, T., Kijima, T., Kishimoto, T., Hosoe, S. Cloning and tissue expression of cDNAs from chromosome 5q21-22 which is frequently deleted in advanced lung cancer. Hum. Genet. 102: 63-68, 1998.

Uniprot ID: O94817

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight: 94973 MW

Gene ID: 9140

Protein Name: Ubiquitin-like protein ATG12

Gene Full Name: autophagy related 12

Synonyms: APG12; APG12 like; APG12L; ATG12; Autophagy related protein 12; FBR93; HAPG12; Ubiquitin like protein ATG12