Anti-Acid phosphatase/ACP5 Antibody Picoband®

SKU: A03277-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ACP5

Specificity:

Background: Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.

Immunogen: E.coli-derived human Acid phosphatase/ACP5 recombinant protein (Position: N39-P325).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Allen, B. S., Ketcham, C. M., Roberts, R. M., Nick, H. S., Ostrer, H. Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. Genomics 4: 597-600, 1989. 2. Bilginer, Y., Duzova, A., Topaloglu, R., Batu, E. D., Boduroglu, K., Gucer, S., Bodur, I., Alanay, Y. Three cases of spondyloenchondrodysplasia (SPENCD) with systemic lupus erythematosus: a case series and review of the literature. Lupus 25: 760-765, 2016. 3. Briggs, T. A., Rice, G. I., Adib, N., Ades, L., Barete, S., Baskar, K., Baudouin, V., Cebeci, A. N., Clapuyt, P., Coman, D., De Somer, L., Finezilber, Y., and 19 others. Spondyloenchondrodysplasia due to mutations in ACP5: a comprehensive survey. J. Clin. Immun. 36: 220-234, 2016. Note: Erratum: J. Clin. Immun. 36: 529-530, 2016.

Uniprot ID: P13686

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight:

Gene ID: 54

Protein Name: Tartrate-resistant acid phosphatase type 5

Gene Full Name: acid phosphatase 5, tartrate resistant

Synonyms: ACP5; Tartrate resistant acid ATPase; TR AP; TRACP; TRAP; TrATPase; Type 5 acid phosphatase