Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5)

SKU: M01141

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Monkey, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: YWHAZ

Specificity: No cross reactivity with other proteins.

Background: 14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 zeta/delta, which shares 97.8% amino acid (aa) sequence identity with both mouse and rat 14-3-3 zeta/delta.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide. 2. Nishimura Y, Komatsu S, Ichikawa D, Nagata H, Hirajima S, Takeshita H, Kawaguchi T, Arita T, Konishi H, Kashimoto K, Shiozaki A, Fujiwara H, Okamoto K, Tsuda H, Otsuji E (Apr 2013). Overexpression of YWHAZ relates to tumor cell proliferation and malignant outcome of gastric carcinoma. British Journal of Cancer. 108 (6): 1324–31.

Uniprot ID: P63104

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 6G5

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight:

Gene ID: 7534

Protein Name: 14-3-3 protein zeta/delta

Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta

Synonyms: 14 3 3 protein zeta/delta; 14-3-3; KCIP 1; YWHAZ