Anti-14-3-3 gamma/YWHAG Antibody Picoband®

SKU: A04148-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: YWHAG

Specificity:

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Immunogen: E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: D21-K162).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Autieri, M. V., Carbone, C. J. 14-3-3-Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells. DNA Cell Biol. 18: 555-564, 1999. 2. Cornell, B., Wachi, T., Zhukarev, V., Toyo-oka, K. Overexpression of the 14-3-3-gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex. Neurosci. Lett. 628: 40-46, 2016. 3. Horie, M., Suzuki, M., Takahashi, E., Tanigami, A. Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23. Genomics 60: 241-243, 1999.

Uniprot ID: P61981

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 211344 MW

Gene ID: 7532

Protein Name: 14-3-3 protein gamma

Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma

Synonyms: 14 3 3 protein gamma; 14 3 3GAMMA; 14-3-3; 14-3-3 GAMMA; KCIP 1; YWHAG