GeneBio Systems

AngII ELISA kit (Human)

Name: AngII ELISA kit (Human)
Brand: GeneBio Systems
SKU: CEA005Hu
Price: 897.95 EUR
Availability: InStock

SKU:CEA005Hu

€897,95 EUR

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Quantity

Size: 96Tests

# of Times Cited in literature: 21

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: AngII

Target Full Name: Angiotensin II

Alternative Names: Ang-II; Angiotensin-2

Target Species: Human

Uniprot: P01019

Gene ID: 183

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human AngII / Angiotensin II

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 24.69-2,000pg/mL

Sensitivity: 9.44pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII). No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to AngII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AngII and unlabeled AngII (Standards or samples) with the pre-coated antibody specific to AngII. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AngII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AngII in the sample.

Research Area: Endocrinology;Cardiovascular biology;

References Citing This Product: Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid

Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction

Angiotensin II promotes the progression of human gastric cancer

Cathepsin G activity lowers plasma LDL and reduces atherosclerosis

Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trial

Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial

Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro

Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.

Ginsenoside Rb1 improves cardiac function and remodeling in heart failure.

Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects

Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar.