5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit

SKU: ELK8155

Size:96T

Reactivity:General

Alternative Names:5-HETE

Assay Type:Competitive Inhibition

Sensitivity:3.83 ng/mL

Standard:800 ng/mL

Detection range:12.5-800 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

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