YY1 Monoclonal Antibody

SKU: AN005360L

Size::25μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Shipping:The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Tags/Controls Antibodies

Abbreviation:YY1

Target Synonym:YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1 (YY-1), INO80S

Research Areas:Epigenetics and Nuclear Signaling;Neuroscience;Isotype;Loading Controls

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human;Mouse;Rat

Application:WB

Isotype:IgG2b

Clonality:Monoclonal

Clone NO.:4C7

UNIProt ID:P25490

Accession:

Background:YY1 is a widely distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. This protein is involved in inhibiting and activating a variety of promoters. YY1 can directly introduce histone deacetylase and histone acetyltransferase into the promoter, thereby activating or inhibiting the promoter, so that histone modification can act on YY1. Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.

Concentration:1 mg/mL

Immunogen:Recombinant human YY1 protein expressed by E.coli

Buffer:PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Purification method:Protein A/G Purification

Dilution:WB 1:1000-1:2000

Calculated MW:45 kDa

ObservedMW:65 kDa